Identification and quantification of nucleic acids oncogenic HPV by PCR assays in real time

摘要

A method for the identification and quantification of nucleic acids oncogenic HPV comprising: a) scanning the first line by 5 PCR assays Real time SYBR Green independent I to determine the total viral load and to identify the presence of one or more of the 13 genotypes of high-risk HPV in the sample; wherein the primers used in step a) having the sequences SEQ ID NO: 1-8; b) testing the second line to apply to samples which have proved positive in exploring first line: - PCR assays Real-time TaqMan independent for the presence of viral load of HPV types most common oncogenic: types HPV: 16, 18, 31, 45, group 33 (including genotypes 33, 52, 58, 67) wherein the primers used in the PCR assay Real Time TaqMan having the sequences SEQ ID 3, 4, 7 , 8, 21-31, - 6 PCR assays Real time SYBR Green I independent RT to determine the presence in the sample of oncogenic transcripts E6 / E7 of HPV types 16, 18, 31, 33, 45, 58 wherein the primers used in the PCR assay Real time SYBR Green in step b having the sequences SEQ ID 9-20.