Composition of culture medium for culturing mesenchymal stem cells derived from amnion and method for culturing mesenchymal stem cells derived amnion using the same

摘要

A medium composition for culturing mesenchymal stem cells, the medium comprising: (i) DMEM-P containing DMEM-HG, 0.2 mM L-ascorbic acid 2-phosphate, fetal bovine serum 10%, 10 ng / ml growth factor basic fibroblast (b-FGF), 10 .mu.l / ml of nonessential amino acid (NEAA); and (ii) P-KSFM containing Keratinocyte-SFM defined, 0.2 mM L-ascorbic acid 2-phosphate, 5 .mu.g / ml insulin, 2 mM N-acetyl-L-cysteine, 0.09 mM calcium chloride, 74 ng / ml hydrocortisone, fetal bovine serum 5%, wherein the non-essential amino acid (NEAA) is 750 mg / L glycine, 890 mg / L L-alanine, 1.320 mg / L L-asparagine, 1.330 mg / L of L-aspartic acid, 1.470 mg / L of L-glutamic acid, 1.150 mg / L of L-proline and 1.050 mg / L of L-serine, and the volume ratio of DMEM -P: KSFM-P is 2: 1, 1: 1 or 1: 3.