Method for producing retinal progenitor polarized cells from pluripotent stem cells and their differentiation to give retinal pigment epithelial cells

摘要

Method for differentiating pluripotent stem cells into polarized progenitor cells retinal, comprising the steps: a) culturing colonies of pluripotent stem cells in three-dimensional cell culture included in a protein gel comprising at least three proteins selected from the group consisting of laminin, collagen IV, entactin and perlecan and comprising at least two, preferably at least three, most preferably at least four factors selected growth EGF, FGF2, NGF, PDGF, IGF-1 and TGF-beta until polarized neural cysts develop containing a single light and consist of polarized cells, b) dissociating polarized cysts neural progenitor cells to give retinal polarized dispersed.