IMMUNOGENIC CONSTRUCTS USEFUL FOR TREATING MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS (MAP) INFECTION

摘要

The present invention relates to molecules, which can be used to induce atherapeutic or prophylactic immune response against MAP. In particular, thepresent invention relates to polypeptides comprising an alipC polypeptidesequence, a gsd polypeptide sequence, a pl2 polypeptide sequence and an mpapolypeptide sequence, wherein said ahpC polypeptide comprises the sequence ofSEQ ID NO: 2, a variant thereof having more than 70% amino acid sequenceidentity to SEQ ID NO: 2 across the full length of SEQ ID NO: 2, or a fragmentof at least 8 amino acids of SEQ ID NO: 2 which comprises an epitope; said gsdpolypeptide comprises the sequence of SEQ ID NO: 6, a variant thereof havingmore than 70% amino acid sequence identity to SEQ ID NO: 6 across the fulllength of SEQ ID NO: 6, or a fragment of at least 8 amino acids of SEQ ID NO:6 which comprises an epitope; said pi 2 polypeptide comprises the sequence ofSEQ ID NO: 10, a variant thereof having more than 70% amino acid sequenceidentity to SEQ ID NO: 10 across the full length of SEQ ID NO: 10, or afragment of at least 8 amino acids of SEQ ID NO: 10 which comprises anepitope; and said mpa polypeptide comprises the sequence of SEQ ID NO: 14, avariant thereof having more than 70% amino acid sequence identity to SEQ IDNO: 14 across the full length of SEQ ID NO: 14, or a fragment of at least 8amino acids of SEQ ID NO: 14 which comprises an epitope. Preferably such avariant maintains the ability to generate an immune response against theunmodified polypeptide.