N-Glycans on IgG As Biomarkers for Autoimmune Diseases Explored Via Comprehensive Glycomic Approach

摘要

#$%^&*AU2015100100A420150312.pdf#####ABSTRACT Described herein as a method of using microfluidic porous graphitized carbon (PGC) chip-LC-MS-based comprehensive glycomic approach to explore N-glycan biomarkers of several kinds of autoimmune diseases. At least two sets of biomarkers may be identified from serum IgG of patients with the autoimmune diseases. The method includes the steps of isolating IgG from serum samples of patients by Protein A, releasing N-glycans from isolated IgG, separating N-glycans from non-glycan compounds by the chip-LC and analyzing the separated N-glycans by mass spectrometry, screening and identifying acidic N-glycans by comparing deconvoluted experimental masses with theoretical N-glycan masses. 13PCA PLS-DA OPLS-DA 3-. 6H 4. 4 4 -5 -6 .4 H 222 111 1111 .- rc S OL0 0.- P 0 1 Ip00 . 6- 1.-02 OA .50.0- Cont ol 0. - Co trolRA 00-C o n tro l R 0-- p0.5 p 0.0 05 pp~ 0.01 0.4 1. 0 30.3- .030 o0.2 = a-a ConrlCntolI oto RA 30333212a 331b 0. p 0.0150 O 00 00 213 aa11b33 0.5-00.0 p.0c0.05 R p0~t . 0 RA ________R a K .. Figure 2 0.4 K40 14