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RAMAN SPECTRAL IMAGE ACQUISITION SYSTEM, AND RAMAN SPECTRAL IMAGE ACQUISITION METHOD

机译:拉曼光谱图像采集系统及拉曼光谱图像采集方法

摘要

[Problem] To obtain a bright field image which can be easily and accurately overlaid on a Raman spectral image. [Solution] A subject (S) is irradiated with excitation light (L0), and the light emitted from the subject (S) due to the irradiation with the excitation light (L0) is spectroscopically measured by a spectrometer (12). The initial light intensity value of the excitation light wavelength from the light that has been emitted from the subject (S) and has entered the spectrometer (12) is adjusted at most 80% of the saturation light intensity of the spectrometer (12). When the light intensity of the excitation light wavelength that enters the spectrometer (12) has been adjusted, the irradiation position of the excitation light (L0) is two-dimensionally scanned, and a bright field image of the subject (S) is obtained from data for the excitation light wavelength of each spectrum measured while two-dimensionally scanning the excitation light (L0). A Raman spectral image of the subject (S) is obtained from Raman-scattered light peak wavelength data, and the bright field image and Raman spectral image are overlaid and displayed on a monitor (3).
机译:[问题]获得可以容易且准确地叠加在拉曼光谱图像上的明场图像。 [解决方案]用激发光(L 0 )照射被检体(S),并通过激发光(L 0 <通过分光计(12)进行分光测定。从被摄体(S)发射并进入光谱仪(12)的光的激发光波长的初始光强度值被调整为光谱仪(12)的饱和光强度的至多80%。调整入射到光谱仪(12)的激发光波长的光强度后,对激发光(L 0 )的照射位置进行二维扫描,得到明场像。从在二维扫描激发光(L 0 )时测量的每个光谱的激发光波长的数据中获得被摄体(S)。从拉曼散射的光峰值波长数据获得被摄体(S)的拉曼光谱图像,并且将亮场图像和拉曼光谱图像叠加并显示在监视器(3)上。

著录项

  • 公开/公告号WO2015068389A1

    专利类型

  • 公开/公告日2015-05-14

    原文格式PDF

  • 申请/专利权人 FUJIFILM CORPORATION;

    申请/专利号WO2014JP05576

  • 发明设计人 YAMAZOE SHOGO;NAYA MASAYUKI;SHIOTA MEGUMI;

    申请日2014-11-05

  • 分类号G01N21/65;

  • 国家 WO

  • 入库时间 2022-08-21 15:06:41

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