METHANE-TO-ACETATE PATHWAY FOR PRODUCING LIQUID BIOFUELS AND BIORENEWABLES

摘要

The engineering of a pathway converting CH₄ to acetate and eventually to liquid fuels is disclosed. The engineered pathway involves an engineered reversal of the natural pathway for acetate conversion to CH₄ by microbes and coupling the engineered pathway to existing and future technologies for microbial or chemical conversion of acetate to liquid fuels. In one aspect, methods for producing modified pathways and/or microbes are provided. In another aspect engineered microbes, including Methanosarcina acetivomns, that incorporate the engineered pathway are provided, which can mediate conversion of CH4 to acetate for ultimate conversion to liquid fuels. In a further aspect, specific modifications to the components of the pathway are provided.