首页> 外国专利> LIGNOCELLULOSE-DEGRADANT-RESISTANT MICROORGANISM AND METHOD FOR PRODUCING SAME, AND METHOD FOR PRODUCING BIOETHANOL USING SAID MICROORGANISM

LIGNOCELLULOSE-DEGRADANT-RESISTANT MICROORGANISM AND METHOD FOR PRODUCING SAME, AND METHOD FOR PRODUCING BIOETHANOL USING SAID MICROORGANISM

机译:耐木质纤维素降解的微生物及其制备方法,以及利用所述微生物制备生物乙醇的方法

摘要

Provided is a microorganism having resistance to a lignocellulose degradant which is a factor that inhibits the fermentation of wood. One embodiment of the present invention is a lignocellulose-degradant-resistant microorganism expressing a higher level of a gene that encodes protein (1) or (2) than does the wild type. A gene that encodes a protein represented by SEQ ID NO: 1 among these genes is a SUMO gene known as an SMT3 gene in yeast. (1) At least one protein selected from the group comprising proteins that comprise amino acid sequences represented by SEQ ID NOS: 1-4; (2) at least one protein selected from the group comprising proteins that comprise amino acid sequences in which one or several amino acids in the amino acid sequences represented by SEQ ID NOS: 1-4 have been deleted, substituted, or added, the amino acid sequences being modified within the range of 80% or higher identity with the amino acid sequence of the corresponding SEQ ID NO., and that have a function for imparting lignocellulose-degradant resistance to microorganisms.
机译:提供了对木质纤维素降解物具有抗性的微生物,木质素纤维素降解物是抑制木材发酵的因素。本发明的一个实施方案是表达比野生型更高水平的编码蛋白(1)或(2)的基因的木质纤维素降解抗性微生物。在这些基因中,编码由SEQ ID NO:1表示的蛋白质的基因是在酵母中被称为SMT3基因的SUMO基因。 (1)至少一种蛋白质,其选自包含具有SEQ ID NOS:1-4所示的氨基酸序列的蛋白质的组; (2)选自包含以下氨基酸序列的蛋白质的组中的至少一种蛋白质,在该氨基酸序列中,由SEQ ID NOS:1-4表示的氨基酸序列中的一个或几个氨基酸已被缺失,取代或添加,在与相应SEQ ID NO。的氨基酸序列具有80%或更高的同一性的范围内被修饰的氨基酸序列,并且具有赋予微生物木质纤维素降解抗性的功能。

著录项

  • 公开/公告号WO2015079868A1

    专利类型

  • 公开/公告日2015-06-04

    原文格式PDF

  • 申请/专利权人 SAGA UNIVERSITY;

    申请/专利号WO2014JP79090

  • 申请日2014-10-31

  • 分类号C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12P7/10;

  • 国家 WO

  • 入库时间 2022-08-21 15:06:15

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