Cis-epoxysuccinate hydrolase-encoding gene, polypeptide encoded by the gene, and related application thereof

摘要

The present invention relates to a cis-epoxysuccinate hydrolase-encoding gene, a polypeptide encoded by the gene and related application thereof. The cis-epoxysuccinate hydrolase-encoding gene has a nucleotide sequence as shown in SEQ ID NO.1, and cis-epoxysuccinate hydrolase encoded by the gene has an amino acid sequence as shown in SEQ ID NO.2. A prokaryotic expression vector constructed by the cis-epoxysuccinate hydrolase gene is transformed into Escherichia coli to construct genetically engineered bacteria which can be induced to efficiently convert cis-epoxysuccinic acid or its salts into L(+)-tartaric acid or its salts. The cis-epoxysuccinate hydrolase produced from E. coli has the ability to efficiently convert cis-epoxysuccinic acid or its salts into L(+)-tartaric acid or its salts.