BIOORTHOGONAL CHEMICAL INDUCERS OF REVERSIBLE DIMERIZATION FOR CONTROL OF PROTEIN FUNCTION IN CELLS

摘要

The present application refers to bioorthogonal chemical inducers of reversible dimerization for control of protein interactions in cells. A compound, a test system, methods and uses are disclosed how the invention can be applied in the investigation of intracellular protein interactions. The system is composed of SLF'-TMP as the dimerizer, displaying a high affinity binding to FKBP(F36V) mutant and eDHFR, respectively. The system is reversible on addition of TMP.