USE OF A REWIRED GENETIC CONTROL NETWORK TO INCREASE PRODUCT EXPRESSION

摘要

A method of identifying a cell with increased production of a desired product, the method comprising: (a) providing a population of cells which produce the desired product; (b) introducing a plurality of distinct nucleic acid molecule species into the population of cells, wherein the nucleic acid molecules comprise a first region which is operably-linked to a second region, wherein the first region comprises a promoter region from a first gene, and the second region comprises a nucleic acid sequence from a second gene that is capable of expressing a regulatory product under control of the promoter region; (c) culturing the population of cells under conditions in which the cells express the regulatory product under control of the promoter region; and (d) testing the population of cells for production of the desired product, thereby to identify a cell or cells with increased production of the desired product. The method may further comprise isolating a cell or cells with increased production of the desired product. The method may further comprise repeating steps (b), (c) and (d) on the isolated cells or cells, or descendants thereof with increased production of the desired product, thereby to identify a cell or cells with further increased production of the desired product. Typically the desired product is not encoded by the introduced nucleic acid molecule. The plurality of distinct nucleic acid molecule species typically comprise a plurality of combinations of multiple first and multiple second regions. The expressed regulatory product typically is a polypeptide but may be an RNA molecule. The method may further comprise isolating the nucleic acid molecule(s) introduced into the cell or cells with increased production of the desired product.