首页> 外国专利> METHOD FOR COMPLETE TRACKING OF A SET OF BIOLOGICAL SAMPLES CONTAINING DNA OR RNA THROUGH MOLECULAR BARCODE IDENTIFICATION DURING LABORATORIAL WORKFLOW AND KIT FOR COLLECTING BIOLOGICAL SAMPLES CONTAINING DNA OR RNA

METHOD FOR COMPLETE TRACKING OF A SET OF BIOLOGICAL SAMPLES CONTAINING DNA OR RNA THROUGH MOLECULAR BARCODE IDENTIFICATION DURING LABORATORIAL WORKFLOW AND KIT FOR COLLECTING BIOLOGICAL SAMPLES CONTAINING DNA OR RNA

机译:在实验室工作流程和试剂盒中通过分子条形码鉴定完整跟踪包含DNA或RNA的一组生物样品的方法以收集包含DNA或RNA的生物样品

摘要

The present invention discloses a method for complete tracking of a set of biological samples containing DNA or RNA through the use of molecular barcode identification during the course of laboratorial workflow, wherein the sample is contacted with primers comprising a molecular barcode and a universal primer sequence immediately after its collection and the amplification of the target regions occurs concomitantly to the barcode insertion, in one or both ends of said target region, in the same annealing temperature through a one step PCR. Finally, the invention also discloses kits for collecting biological samples containing DNA or RNA.
机译:本发明公开了一种在实验室工作流程过程中通过使用分子条形码识别来完全跟踪一组含有DNA或RNA的生物样品的方法,其中样品立即与包含分子条形码和通用引物序列的引物接触。在收集后,目标区域的扩增伴随条形码的插入,通过一步PCR在相同退火温度下在所述目标区域的一端或两端进行。最后,本发明还公开了用于收集含有DNA或RNA的生物样品的试剂盒。

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