The present invention relates to a zebrafish-based method for screening an anticancer agent, the method comprising the steps of (a) injecting and grafting human cancer cells into yolk sacs of zebrafish (Danio rerio); (b) contacting the product in step (a) with a test material; (c) culturing the product in step (b) at 30-33C; and (d) counting zebrafish with disseminated human cancer cells at 3-9 days per injection (dpi), and here, the test material is determined to be an anticancer agent when the number of zebrafish is decreased by at least 10% when compared with the control group. According to the present invention, an optimum screening system using a xenograft model in which human cancer cells are injected into zebrafish, thereby promptly and conveniently detecting candidate anticancer materials. Furthermore, a triazine-based compound screened by the method of the present invention reduces the dissemination, infiltration, or viability of cancer cells.
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