A PHARMACEUTICAL COMPOSITION FOR PREVENTING OR TREATING ARTHRITIS COMPRISING HYALURONIC ACID-METHOTREXATE CONJUGATE AND A METHOD FOR PREPARATION THEREOF

摘要

A purpose of the present invention relates to a pharmaceutical composition including a compound represented by chemical formula 1 as an active ingredient for preventing or treating arthritis, a method for producing a compound of chemical formula 1 comprising a step of reacting methotrexate with hyaluronic acid under the presence of 4-dimethylaminopyridine and N,N;-dicyclohexylcarbodiimide, and a hyaluronic acid-methotrexate conjugant represented by chemical formula 1. The HA-MTX conjugant of the present invention can discharge MTX when an ester bond is hydrolyzed in an acidic condition, so that a separated enzyme is not needed. Even when the HA-MTX conjugant is systemically administered by means of an intravenous injection, a drug is selectively discharged from an arthritic region which is lower than normal tissues so that side effects are minimized and the HA-MTX conjugant can be effectively used as an arthritis treating agent.