Preparation method of micro hemisphere array plate microfluidics chip comprising micro hemisphere array plate and culture method of cell spheroid using the same

摘要

The present invention relates to a method of manufacturing a microsphere array plate, a microfluidic device including a microsphere array plate, and a method of culturing cell aggregates using the same. When the cells are three-dimensionally cultured using the microfluidic array plate according to the present invention, the microfluidic device including the microsphere array plate, and the method of culturing the cell aggregate using the microfluidic array plate, Lt; / RTI In particular, since the cells are cultured under similar conditions to the environment in the human body, it is possible to form the cell aggregate in a better state than the existing cell culture methods. The cell aggregate cultured through the present invention can be directly used for cell therapy and can be obtained by culturing cells that are difficult to obtain artificially. In addition, since it is cultured by providing conditions similar to human body, it is possible to form cell aggregates of cells which are difficult to be aggregated in three dimensions. In addition, it is possible to co-culture two or more cells, and at this time, it is possible to form a cell cluster of excellent quality because it is cultured under conditions similar to human body. CONCLUSION Through the present invention, dramatic improvements in drug screening, cytotoxicity, and various tests can be achieved.