NOVEL DNA CAPABLE OF BEING AMPLIFIED BY PCR WITH HIGH SELECTIVITY AND HIGH EFFICIENCY

摘要

the present invention, high selectivity and high efficiency in the replication as Ds (5 - amino 7- (2-thienyl)-3H-imidazo [4,5-b] pyridin-3-yl)-PA derivative (2-nitro-bound at the 4-position a substituent of the electron system -1H- pyrrol-1- It relates to a method of cloning a nucleic acid comprising the artificial base pairs, and the art of artificial base pairs in the group). The invention also to the functional substituents bound to a method of artificial base introduced into DNA by a nucleic acid replication reaction. The invention also replicate the nucleic acid from a nucleic acid pool including the artificial base pairs, to a method for selectively recovering. The present invention, further addition, high efficiency of the nucleic acid containing the artificial base and will also relates to a method for determining the nucleotide sequence of the native DNA in the vicinity of the artificial base to realize high selective replication.