Method of Production of Transgenic Avian using Embryonic Stem Cells

摘要

the present invention includes: a) non-modified cultures of embryonic birds know the origin of ES cells vigor disk the insulin-like growth factor -1 (IGF-1) and ciliary neurotrophic factor (CNTF); And animal serum; And, optionally, interleukin-6 (IL-6), interleukin 6 receptor (IL-6R), stem cell factor (SCF), fibroblast growth factor (FGF), leukemia inhibitory factor (LIF), interleukin 11 (IL-11) the step of suspending the oncostatin and Cardio basic culture medium containing the Trojan pin; b) culturing for at least 2-10 passages the ES cells after inoculating the ES cell suspension feeder layer obtained in step a); c) SCF, FGF, IL-6, IL6R, selectively removing from the LIF, oncostatin and cardiolipin Trojan pin and at least one IL-11 in the culture medium the growth factor is selected from; d) relates to avian embryonic stem (ES) cell cultures comprising culturing the ES cells further in feeder layer in the medium of step c).