DNA encoding the dipeptide-synthesizing enzyme (OPTIONS), bacteria GENUS ESCHERICHIA AND METHOD FOR ITS USE dipeptide

摘要

1. DNA encoding a protein having dipeptide-synthesizing activity, wherein said DNA is selected from the group consisting of: (A) a DNA having a nucleotide sequence of SEQ ID NOs: 1, 3 and 5; (B) a DNA hybridizing under stringent conditions with a nucleotide sequence complementary to the sequence shown in SEQ ID NOs: 1, 3 and 5, characterized in that said stringent conditions include washing one or more times in a solution containing a salt concentration of 1 × SSC, 0,1% SDS or 0,1 × SSC, 0,1% SDS at 60 ° C or 65 ° C; (C) DNA encoding a protein having the amino acid sequence of SEQ ID NOs: 2, 4, and 6; (D) DNA encoding a protein variant having the amino acid sequence of SEQ ID NOs: 2, 4 and 6, but contains a substitution, deletion, insertion or addition of one or several amino acid residues, and having dipeptide-synthesizing activity in accordance with the amino acid sequence of SEQ ID NOs: 2, 4, and 6; (E) DNA encoding a protein having a homology determined by the value of | Log (E-value) |, is not less than 128 not less than 142 not less than 162 not less than 175 not less than 182, at least 196, or at least than 233 compared to the amino acid sequence of SEQ ID NOs: 2, 4 and 6, and having dipeptide-synthesizing activity in accordance with the amino acid sequence of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16 and 18.2. The recombinant DNA for expression of the DNA of claim 1, comprising the DNA according to paragraph 1.3. The dipeptide-producing bacterium belonging to the genus Escherichia and modified so that it contains a recombinant DNA according to item 2.4. The bacterium according to claim 3, wherein the bacterium belongs to Escherichia coli.5 mind. The bacterium according to any one of claims 3 or 4, characterized in that the decree