Process for the preparation of antigen-specific cytotoxic cells with activity against BREAST CANCER CELLS

摘要

1. A method for producing antigen-specific cytotoxic cells having anti-tumor activity against breast cancer cells, comprising the following steps: a) isolating mononuclear cells (MNC) from peripheral blood of a patient, and b) cultivating MNCs division adhering cells and non-adherent fraction, in ) growing adherent MNC fraction in the presence of growth factors to obtain mature dendritic cells (DC), d) transfection of mature DC with expression plasmids containing a DNA vaccine constructs for diruyuschimi versatile and HLA-A * 0201-specific epitopes major tumor-associated antigens recognized by cytotoxic T lymphocytes (CTL) and T-cell helper (Th); d) co-culturing of mature DC obtained in step d) and nonstick MNC fraction in the presence of recombinant human IL-12 and recombinant human interleukin-18.2. A method according to claim 1, characterized in that as growth factors for growing adherent MNC fraction using granulocyte macrophage colony-stimulating factor (GM-CSF) and human interleukin-4 (IL-4) human.