METHOD FOR DETERMINING THE STABILITY C3 convertase of the classical pathway of complement activation RIGHTS

摘要

A method of determining stabilization of C3-convertase of the classical pathway of complement activation system through the control and experimental samples reactions lysing erythrocytes human serum at a temperature of 37,0 ± 0,5 ° C, followed by detection of lysis of erythrocytes, characterized in that the lysis of sheep red blood cells is performed 0.8% human serum for 10 min, quenched with buffer containing 10 mM EDTA, determine the degree of lysis of red blood cells in the control sample, the test samples were further incubated for 30 min at 37 ° C and determine the degree of lysis of erythrocytes, and C3-convertase activity is calculated as the difference between the degrees of lysis in the test and control samples, the difference of more than 10% is evaluated as autoimmune pathological condition.