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METHOD seedlings PINE (Pinus sylvesrtis L.) and nutrient medium used in this process
METHOD seedlings PINE (Pinus sylvesrtis L.) and nutrient medium used in this process
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机译:方法在该过程中使用松树樟子松(Pinus sylvesrtis L.)和营养培养基
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摘要
1. A method of growing seedlings of Scots pine (Pinus sylvesrtis L.), consists in the fact that the seeds of Scots pine, washed in distilled water, placed in a polypropylene sleeve, provided with wads of cotton, fixed in the holes of the raft of polystyrene, which is positioned on the surface of the liquid nutrient medium in a climatic chamber, dichromatic electroluminescent illuminated with light of wavelength 460 nm and 580 nm and the intensity of the luminous flux 140-200 micromole quanta per ms, at a temperature of 25 ° C, humidity 60%, with replacement of the culture medium once per 72 hours and its air forced aeration at 15 liters per chas.2. The medium used in the method of claim 1 performed in the following ratio: NHNO- 2,0 mM; KHPO- 1,5 mM; MgSO- 0,5 mM; NaSO- 0,1 mM; CaCl- 1,0 mM; HBO- 55 uM; MnSO- 5 uM; ZnSO- 1,26 M; CuSO- 0,32 M; NaMoO- 0,1 M; Co (NO) - 0,02 M; KI - 1,0 M; FeSo- 9,5 M; sodium ethylenediaminetetraacetate - 9.5 M; pH = 4,5.
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机译:1.一种生长樟子松(Pinus sylvesrtis L.)的方法,其特征在于,将樟子松的种子用蒸馏水洗净,放在聚丙烯套中,在聚丙烯套中固定一团棉花,固定在棉花的孔中。放置在气候室内液体营养培养基表面上的聚苯乙烯筏,用波长460 nm和580 nm的光以及140-200微摩尔量子/ ms的强度照亮二色电致发光温度为25°C,湿度为60%,每72小时更换一次培养基,并以每升15升的压力进行空气充气。2。权利要求1的方法中使用的介质以以下比例进行:NHNO-2,0mM; KHPO- 1.5毫米;硫酸镁-0.5 mM; NaSO-0.1 mM; CaCl- 1.0 mM; HBO- 55 uM; MnSO-5 uM;硫酸锌1,26 M;硫酸铜-0.32 M; NaMoO-0,1 M; Co(NO)-0,02 M; KI-1,0 M; FeSO-950万;乙二胺四乙酸钠-9.5 M; pH = 4.5。
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