METHOD FOR PRODUCING AND haploid dihaploid BARLEY PLANTS USING CULTURE microsporia

摘要

1. A method for producing haploid dihaploid and barley plants from cultured microspores in vitro, comprising: A. cultivation of donor plants under certain conditions to a stage optimum for isolating and culturing microspores to induce embryogenesis and plant regeneration; B. stressful handling ears (low temperature + poor medium with mannitol, isolation of microspores in poor refrigerated environment) to switch the path from gametophytic development of microspores in sporophytic path; B. culturing the isolated microspores in modified medium for induction of microspore embryogenesis (growth regulators, maltose and ovaries); T. Plant regeneration from the embryos on a solid medium; D. doubling the chromosomes in the haploid plants using antimitotic preparatov.2. A method according to claim 1, characterized in that the donor plants grown under defined conditions (vernalization seeds and seedlings, low temperature 15-20 ° C air, light regime: 16 h day / 8 - night, 60-70% humidity, light intensity 10000-15000 lux) with increased control of phytopathogenic aktivnosti.3. A method according to claim 2, characterized in that for determining the developmental stage of microspores not conducted cytological research and development stage is determined by the stage of development of the donor plant, wherein the microspores are used to isolate the plant at the stage when the flag leaf sheath was opened, but another inflorescence It is within lista.4 flag. A method according to claim 1, characterized in that, for switching from gametophytic development of microspores in sporophytic path using low temperature processing of freshly ears rasteniy-