METHOD OF IMPROVING OF THE CATALYTIC PROPERTIES OF PENICILLIN ACYLASE FROM ESCHERICHIA OF COLI AND USE OF MUTANT PENICILLIN ACYLASE

摘要

1. A method for improving the catalytic properties of the Escherichia coli penicillin acylase in reactions stereoselective acylation of primary amino groups of chemical compounds selected of the group consisting of amines, amino alcohols, amino acids and their amides and esters, and the stereoselective hydrolysis of N-acyl derivatives of primary amine compounds selected of the group consisting of esters, amides, O- or N-acylated derivatives of alcohols, amines, aminoalcohols, amino acids, their amides and esters, comprising changing said penicillin acylase is replaced by the structure 145 s of an amino acid residue on the alpha chain amino acid residue leucine or beta chain 71 to leucine or arginin.2. Use of the mutant penicillin acylase from Escherichia coli, wherein the amino acid residue 145 of the alpha chain is replaced by leucine or amino acid residue 71 beta-chain is replaced by leucine or arginine for the stereoselective acylation of primary amino groups of chemical compounds selected from the group consisting of amines, amino alcohols, amino acids, and stereoselective hydrolysis of N-acyl derivatives of primary amine compounds selected from the group consisting of esters, amides, O- or N-acylated derivatives of alcohols, amines, aminoalcohols, amino acids, their amides and esters.