The separation and purification of recombinant human prourokinase M5

摘要

1. A method for isolating prourokinase M5 of inclusion bodies containing this prourokinase comprising a stage of destruction of the inclusion bodies, refolding and purification of the protein, characterized in that before the renaturation is carried out by chromatography on protein pretreatment Q-Sepharose and SP-Sepharose columns using combined with Q - and SP-Sepharose and, after renaturation is carried chelating and ion exchange chromatography of recombinant prourokinase M5 without intervening elution of the target protein using metallhelatnogo sorbent activated UQ We Soili Zn.2. A method according to claim 1, characterized in that the sorbent is metallhelatny IMAC-Sepharose or Chelating-Sefarozu.3. Application metallhelatnogo sorbent activated ions Soili Ζn, for chelating and ion exchange chromatography of recombinant prourokinase M5 without intermediate target belka.4 elution. Use according to claim 3, characterized in that the sorbent is metallhelatny IMAC-Sepharose or Chelating-Sepharose.