METHOD OF DIAGNOSIS automated morphometric myelofibrosis

摘要

1. A method for automated morphometric diagnostic myelofibrosis, comprising executing overview images of the biological tissue, selection in images such zones with different optical properties, defined by fibrous and hematopoietic properties of biological tissues and perform calculation of the ratio of areas of these zones, characterized in that the overview image is performed by forming both at least three paraffin sections trepanobioptate obtained sections were treated as follows: first impregnated Ser bromine by the method of Gomori second stained by the method of Van Gieson and third stained with hematoxylin and eosin thus obtained scenic images is subjected to fixation and analysis so that as a result of this analysis was obtained from the amount of area zones colored in black and pink colors corresponding fibrous tissue and value of the area zones colored in blue and pale pink color, corresponding to the hematopoietic tissue; then calculated the relative area ratio as a ratio of the area of ​​fibrous tissue in the area on the hematopoietic tissue formula: where K is the relative area ratio (in percentage); S- area tissue area painted black; S- area tissue area stained in pink ; S- tissue zone area colored in pale pink color; S- tissue zone area colored in blue color, wherein at a value K≥14,5% mielofibroz.2 diagnosed. Automated morphometric method according to claim diagnostic myelofibrosis. 1, characterized in that the paraffin sections trepanobioptate mkm.3 4 is formed thick. A method of automated morphometric diagnostic Mie