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TRANSGENIC PLANT OSEENS VERSATILE BY PRODUCTIVITY AND BY MODIFIED WOOD

机译:按生产力和改性木材分类的转基因植物

摘要

1. A transgenic plant with increased productivity aspen and the modified timber and its progeny of sexual and / or asexual, resulting unique transformation act between a nucleic acid molecule encoding glutamine synthetase, and genomic DNA osiny.2. The transgenic plant according to claim 1, wherein the nucleic acid molecule is a cDNA molecule isolated from Scots pine (Pinus sylvestris L.), having the nucleotide sequence shown in SEQ ID N0:. 1.3. The transgenic plant according to claim. 1, where aspen is the representative species Populus tremula L.4. The transgenic plant according to claim 1, which exhibits one or more of the following signs of increased productivity, but not limited to:. An increased growth rate, increased biomass yield, larger leaves and increased activity of glutamine synthetase, increased nitrogen use efficiency as compared to the wild type counterpart. 5. The transgenic plant according to claim 1, which exhibits one or more of the following features wood modification, but not limited to:. Altered lignin content, altered cellulose content, modified hemicellulose content, a modified structure of lignin, modified parameters wood fibers as compared with an analogue wildtype. 6. A method for producing a transgenic plant aspen according to claim 1 comprising:.. A) Agrobacterium-mediated transformation of explants of aspen in vitro a nucleic acid molecule having the nucleotide sequence of claim 2, and b) regeneration of plants from transformed explants cells, c) identifying the nucleic acid molecule encoding a glutamine synthetase
机译:1.转基因植物,具有提高的白杨生产力和改性木材及其有性和/或无性后代,从而在编码谷氨酰胺合成酶的核酸分子与基因组DNA osiny之间产生独特的转化作用。2。 2.根据权利要求1所述的转基因植物,其中,所述核酸分子是从SEQ ID NO:1所示的核苷酸序列的苏格兰松(Pinus sylvestris L.)分离的cDNA分子。 1.3。 7.根据权利要求1的转基因植物。 1,其中白杨是代表种杨白杨L.4。 2.根据权利要求1所述的转基因植物,其表现出以下一种或多种提高生产力的迹象,但不限于:与野生型相比,增加的生长速率,增加的生物量产量,更大的叶片和谷氨酰胺合成酶的活性增加了氮的利用效率。 5.根据权利要求1所述的转基因植物,其具有以下一种或多种木材改性的特征,但不限于:与类似的野生型相比,木质素含量改变,纤维素含量改变,半纤维素含量改变,木质素结构改变,木纤维参数改变。 6.根据权利要求1所述的生产转基因植物白杨的方法,包括:。A)农杆菌介导的白杨外植体转化具有权利要求2的核苷酸序列的核酸分子,和b)从转化的植物再生外植体细胞,c)鉴定编码谷氨酰胺合成酶的核酸分子

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