KINETIC METHOD FOR TESTING OF ANTIOXIDANTS

摘要

Kinetic test method antioxidants by saturating the sample esters of higher unsaturated fatty acids and antioxidants oxygen with stirring at a temperature of 60,0 ± 0,2 ° C and the formation of oxidized radicals circuits, determining the amount of oxygen absorbed with time by plotting a dV / dt coordinates, consisting of a model substrate, comprising the esters of higher unsaturated fatty acids, an aqueous solution of cetyltrimethylammonium bromide with final concentration of (1-5) × 10mol / L, the copper salt (II), or a sample of the solution was anti- ksidanta, the feature is that instead of the aqueous solution of salts of copper (II) complex using copper salt (II) with a final concentration of (1-3) × 10mol / LCA α-alanine (or valine) at a final concentration of (5-10) × 10mol / dm prepared in borate buffer at pH (8-10) or an antioxidant is added a sample of the solution, the resulting sample was adjusted to 4 ml of water, the ratio of lipid: water - 1: 3, saturated with oxygen, volumetric method determines the amount of oxygen absorbed in time, plotted in the dV / dt on the basis of coordinates obtained by differentiating x kinetic curves approximated by three portions.