METHOD Biosynthesis of recombinant interferon beta-1a With optimized profile of glycosylation in an industrial environment

摘要

1. A method for the recombinant biosynthesis of interferon-beta-1a in slurry Chinese hamster ovary line CHO-S, containing the gene of human interferon-beta, wherein the cell line culture CHO-S-VKPM H-124 is carried out in serum free medium in the bioreactor wave type using the two-phase temperature regime as follows: 2 days at 37 ° C, 6 days at 30 ° C.2. A method according to claim 1, characterized in that for optimization of the glycosylated forms of recombinant interferon-beta-1a used additive consisting of 2 mM uridine and 10 mM galactose.