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OPTIONS gene promoter GAP, encodes glyceraldehyde-3-phosphate dehydrogenase

机译:OPTIONS基因启动子GAP,编码3-磷酸甘油醛脱氢酶

摘要

1. An isolated polynucleotide having a promoter activity which comprises a polynucleotide having the nucleotide sequence shown in SEQ ID NO: 3 or SEQ ID NO: 34.2. A polynucleotide according to claim 1, characterized in that it consists essentially of a polynucleotide having the nucleotide sequence shown in SEQ ID NO: 3 or SEQ ID NO: 34.3. The polynucleotide of claim 1 or 2, characterized in that it consists of the nucleotide sequence shown in SEQ ID NO: 3 or SEQ ID NO: 34.4. A polynucleotide according to claim 3, characterized in that it is operably linked at its 3 'end directly or via a linker oligonucleotide or polynucleotide via a linker, preferably via a linker polynucleotide to a second polynucleotide that comprises at the 5'-end of the start codon ATG or GTG and encodes one or more polypeptide (s) .5. A polynucleotide according to claim 4, characterized in that the polynucleotide of SEQ ID NO: 3 or SEQ ID NO: 34 through the adenosine nucleotide at position 461 at its 3'-terminus is linked via the linker oligonucleotide consisting of 1, 2, 3 , 4 or 5 nucleotides from the first nucleotide of the start codon of the second polinukleotida.6. A polynucleotide according to claim 4, characterized in that the polynucleotide of SEQ ID NO: 3 or SEQ ID NO: 34 through adenosine nucleotide at its 3 'end is linked via a linker polynucleotide, comprising from 6 to not more than (≤) 600 nucleotides, preferably 20, 21, 22, 23, 24 or 25 nucleotides from the first nucleotide of the start codon of the second polinukleotida.7. A polynucleotide according to claim 6, characterized in that the linker polynucleotide comprises a nucleotide sequence which ensures broadcast RNK.8 synthesized. Floor
机译:1.具有启动子活性的分离的多核苷酸,其包含具有SEQ ID NO:3或SEQ ID NO:34.2所示核苷酸序列的多核苷酸。 2.根据权利要求1的多核苷酸,其特征在于它基本上由具有SEQ ID NO:3或SEQ ID NO:34.3所示核苷酸序列的多核苷酸组成。 3.根据权利要求1或2所述的多核苷酸,其特征在于,其由SEQ ID NO:3或SEQ ID NO:34.4所示的核苷酸序列组成。 4.根据权利要求3所述的多核苷酸,其特征在于,其直接在其3'端或通过接头寡核苷酸或经由接头的多核苷酸,优选地通过接头多核苷酸可操作地连接至在其5'端包含的第二多核苷酸。起始密码子ATG或GTG并编码一种或多种多肽.5。 5.根据权利要求4的多核苷酸,其特征在于,SEQ ID NO:3或SEQ ID NO:34的多核苷酸通过其3′-末端461位的腺苷核苷酸通过由1、2、3组成的接头寡核苷酸连接。第二个polinukleotida的起始密码子的第一个核苷酸的第一个,第4个或第5个核苷酸; 6。 5.根据权利要求4的多核苷酸,其特征在于SEQ ID NO:3或SEQ ID NO:34的多核苷酸通过其3'端的腺苷核苷酸通过接头多核苷酸连接,所述接头多核苷酸包含6至不超过(≤)600核苷酸,优选从第二个小核科的起始密码子的第一个核苷酸的第一个核苷酸起的20、21、22、23、24或25个核苷酸。 7.根据权利要求6所述的多核苷酸,其特征在于,所述接头多核苷酸包含确保广播的RNK.8合成的核苷酸序列。地板

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