ISOLATION OF VIRUS RELATED TO PARVOVIRUS-2 DOGS, FROM RACON

摘要

1. An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 or part of SEQ ID NO: 1; wherein said part of SEQ ID NO: 1 encodes an antigenic region of VP-2 protein including amino acid residue 232 of SEQ ID NO: 3, amino acid residue 300 of SEQ ID NO: 3, or both amino acid residue 232 and amino acid residue 300 of SEQ ID NO: 3.2 . An immunogenic composition comprising a nucleic acid according to claim 1, wherein said nucleic acid is present in the virion of a killed or attenuated parvovirus or RPV of low-passaging. 3. The immunogenic composition according to claim 2, wherein said virion of the killed parvovirus includes the characteristics of parvovirus-2 (from a raccoon) with the deposit number in ATCC - PTA-11400.4. The immunogenic composition according to claim 2, wherein said virion of the attenuated parvovirus is present in a solid carrier suitable for supralingual dissolution. The immunogenic composition of claim 2, which is suitable for subcutaneous administration. A method of inducing an animal's immune response against parvovirus infection, comprising the step of administering to said animal an immunogenic composition comprising a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 or part of SEQ ID NO: 1; wherein said part of SEQ ID NO: 1 encodes an antigenic region of VP-2 protein including amino acid residue 232 of SEQ ID NO: 3, amino acid residue 300 of SEQ ID NO: 3, or both amino acid residue 232 and amino acid residue 300 of SEQ ID NO: 3.7. The method of claim 6, wherein said nucleic acid is present in the virion of a killed or attenuated parvovirus or RPV of low-passaging. The method of claim 7, wherein said virion of the killed parvovirus, including