SET OF DIFFERENTIATING NUCLEOTIDES AND BIOCHIP APPLICABLE IN METHOD FOR GENETIC TYPING OF HUMAN Y-CHROMOSOMES HAPLOGROUP MARKERS: M130 (C), M145 (DE)

摘要

FIELD: medicine.;SUBSTANCE: group of inventions refers to genetics, molecular biology, forensic medicine and criminal science, and concerns an instrument for identifying human Y-chromosome haplogroups. The group of inventions is presented by a set of differentiating oligonucleotide probes, a biochip containing these probes, and a method for using the biochip for analysis. The biochip makes it possible to identify 10 haplogroups of human Y-chromosome. The haplogroup is identified by genetic typing of Y-chromosome by 10 markers: M130 (C), M145 (DE), P257 (G), M69 (H), U179 (I), M304 (J), M185 (L), M231 (N), M175 (O) and P224 (R) with the use of the biochip with the immobilised oligonucleotides a sequence of which is specified in table 1.;EFFECT: structure of the differentiating oligonucleotides used in each invention of the group enables providing binding them to complementary targets only, thereby providing a bright fluorescent signal in the respective biochip cells.;3 cl, 2 dwg, 1 tbl, 3 ex