NUTRIENT MEDIUM FOR DETERMINATION OF LIPASE-PRODUCING MICROORGANISMS IN CONFECTIONARY PRODUCTS

摘要

FIELD: food industry.;SUBSTANCE: method involves components selection and preliminary preparation by way of sterilisation with further mixing of the components in equal quantities. The first component of the nutrient medium is a nutrient base containing peptone, sodium chloride, agar and a blue alcohol-based colouring agent, all dissolved in water, at the preset ratio. The second component of the nutrient medium is a fat emulsion sterilised at the preset parameters and consisting of Twin 80 cocoa butter substitute and water mixed in the preset quantities. The nutrient medium and the fat emulsion are mixed after sterilisation either immediately before usage, in equal quantities, with addition of the blue alcohol-based colouring agent in the preset quantity.;EFFECT: invention allows to increase accuracy of lipase-producing microorganisms detection.