METHOD OF MANUFACTURING VACCINE, ASSOCIATED AGAINST PSEUDOMONOSIS AND VIRAL RABBIT HAEMORRHAGIC DISEASE

摘要

FIELD: medicine.;SUBSTANCE: invention relates to method of manufacturing vaccine, associated against pseudomonosis and viral rabbit haemorrhagic disease. Characterised method includes selection of affected organs from dead rabbits in the period of their disease from local epizootic focus, separation of pure cultures of disease causing agents, for which purpose separate growing of cultures Pseudomonas aeruginosa and virus of rabbit haemorrhagic disease if performed. To obtain causative agent of pseudomonosis, Pseudomonas aeruginosa culture is grown in beef-extract broth with addition of glucose. To obtain virus of haemorrhagic disease, rabbits are infected with virulent virus of rabbit haemorrhagic disease with infection activity not lower than 10³ LD 50/cm³. Samples of dead rabbit's liver are taken, its milling and homogenisation performed. Inactivated cultures Pseudomonas aeruginosa and viral rabbit haemorrhagic disease are mixed in equal proportions Solution of aluminium hydroxide is introduced, mixed, packed and sealed.;EFFECT: claimed invention makes it possible to manufacture harmless, highly-immunogenic vaccine, stable in storage.;1 tbl