METHOD OF OBTAINING OF COPOLYMER OF 3-HYDROXYBUTYRATE, 3-HYDROXYVALERATE AND 4-HYDROXYBUTYRATE

摘要

FIELD: biotechnologies.SUBSTANCE: method of obtaining of copolymer of 3-hydroxybutyrate, 3-hydroxyvalerate and 4-hydroxybutyrate is offered. The method includes the cultivation of the strain producer Cupriavidus eutrophus VKPM V-10646 in conditions of aeration and stirring in liquid salt medium. The main carbon substratum is glucose or oleic acid. The liquid salt medium contains yeast extract in the concentration 2 g/l. The additional source of carbon are substrata-predecessors in the form of potassium valerate in the concentration 1.0-2.0 g/l and gamma-butyrolacton in the concentration 1.5-2.5 g/l, and the additional source of carbon is added at the first stage of fermentation process gradually within 1.5-2 h at total duration of the process 32 hours minimum.EFFECT: high yield of copolymer, improvement of physical and chemical, physicomechanical, process properties of polymer and decrease of level of crystallinity of copolymers suitable for obtaining of elastic films.5 dwg, 1 tbl, 5 ex