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A method and pressure - rotary bed - bioreactor used for the dynamic expansion and / or differentiation of stem cells or primary cells, human and animal origin
A method and pressure - rotary bed - bioreactor used for the dynamic expansion and / or differentiation of stem cells or primary cells, human and animal origin
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机译:一种用于人和动物来源的干细胞或原代细胞动态扩增和/或分化的方法和压力旋转床生物反应器
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摘要
A method for dynamic expansion and / or differentiation of stem cells or primary cells, human and animal origin, in addition to embryonic stem cells, wherein the cells on cell carriers or implant under position (5, 5a, 5b) adhere and by means of dynamic supply of nutrients and the gas atmosphere is expanded or differentiated and wherein, in a reactor housing (1) as a support or implant under position (5, 5a, 5b), by means of a magnetic drive driven rotating direction of rotation of the bed (6), is arranged, characterized in that the cell carriers or implant under layers (5, 5a, 5b) dynamically adhered cells are cultivated and a simultaneous constant or continuously increasing or alternating pressure load of equal to / is greater than 1 to 20 bar are subjected to, wherein the cell carrier or implant under position (5, 5a, 5b) by means of which in the reactor housing (1) by means of the culture medium and which is located in the head space of the reactor housing (1) - atmosphere gas which is alternately with a constant rotational speed of the truck bed (6) between 0,1 to 20 rpm and rotatingly moved in this case, the media and the gas atmosphere - to - and be removed continuously.
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