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A method for ex - vivo - cultivation of woven fabrics or embryonic organ systems
A method for ex - vivo - cultivation of woven fabrics or embryonic organ systems
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机译:一种机织织物或胚胎器官系统的离体培养方法
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摘要
A method for ex - vivo - cultivation of woven fabrics or embryonic organ systems, with the exception of the organ systems from human embryos, on a multiwell plate (1), comprising the steps of:a) placing in each case of one or more fabric or of one or more of embryonic organ systems (6) at or near the center of the circular surface of the well bottom (4) in a single well (2);b) addition of the complete nutrient medium (7) in an amount for the complete wetting of the circular surface of the well bottom (4) is necessary; andc) incubating the / of the fabric (s) or of the embryonic organ system (n) (6) and of the nutrient medium (7) in a humid atmosphere enriched with carbon dioxide and a temperature range from 35 to 39°C,characterized in that multiwell plates have (1) of the configuration variables 6 - well (12 - well, 24 - well, 48 - well, 96 - well or 384 - well be used and for the wells (2) of the different well plates formats with the appropriate well bottom surfaces (5) the following volumes / areas of nutrient medium (7) are provided:
Multi well plates formatSize of the well bottom surface (5) in cm2Nutrient medium volume in μl6 Well9,6816 (±20012 Well3,9331,5 (±7024 Well1,9161,5 (±4048 Well1,085 (±30)96 Well0,3227,2 (±10)384 Well0,18,5 (±3).