Multi well plates formatSize of the well bottom surface (5) in cm2Nutrient medium volume in μl6 Well9,6816 (±20012 Well3,9331,5 (±7024 Well1,9161,5 (±4048 Well1,085 (±30)96 Well0,3227,2 (±10)384 Well0,18,5 (±3)."/> A method for ex - vivo - cultivation of woven fabrics or embryonic organ systems
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A method for ex - vivo - cultivation of woven fabrics or embryonic organ systems

机译:一种机织织物或胚胎器官系统的离体培养方法

摘要

A method for ex - vivo - cultivation of woven fabrics or embryonic organ systems, with the exception of the organ systems from human embryos, on a multiwell plate (1), comprising the steps of:a) placing in each case of one or more fabric or of one or more of embryonic organ systems (6) at or near the center of the circular surface of the well bottom (4) in a single well (2);b) addition of the complete nutrient medium (7) in an amount for the complete wetting of the circular surface of the well bottom (4) is necessary; andc) incubating the / of the fabric (s) or of the embryonic organ system (n) (6) and of the nutrient medium (7) in a humid atmosphere enriched with carbon dioxide and a temperature range from 35 to 39°C,characterized in that multiwell plates have (1) of the configuration variables 6 - well (12 - well, 24 - well, 48 - well, 96 - well or 384 - well be used and for the wells (2) of the different well plates formats with the appropriate well bottom surfaces (5) the following volumes / areas of nutrient medium (7) are provided: <TableGroup colSep="0" cols="3" rowSep="0"><Colspec colName="col1" colNumber="1" colWidth="2*"/><Colspec colName="col2" colNumber="2" colWidth="2*"/><Colspec colName="col3" colNumber="3" colWidth="2*"/><Tbody><Row><entry colName="col1">Multi well plates format</entry><entry>Size of the well bottom surface (5) in cm<Sup>2</Sup></entry><entry colName="col3">Nutrient medium volume in μl</entry></Row><Row><entry colName="col1">6 Well</entry><entry colName="col2">9,6</entry><entry colName="col3">816 (±200</entry></Row><Row><entry colName="col1">12 Well</entry><entry colName="col2">3,9</entry><entry colName="col3">331,5 (±70</entry></Row><Row><entry colName="col1">24 Well</entry><entry colName="col2">1,9</entry><entry colName="col3">161,5 (±40</entry></Row><Row><entry colName="col1">48 Well</entry><entry colName="col2">1,0</entry><entry colName="col3">85 (±30)</entry></Row><Row><entry colName="col1">96 Well</entry><entry colName="col2">0,32</entry><entry colName="col3">27,2 (±10)</entry></Row><Row><entry colName="col1">384 Well</entry><entry colName="col2">0,1</entry><entry colName="col3">8,5 (±3).</entry></Row></Tbody></TableGroup></TableFormula></Table> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译:</span>一种在多孔板(1)上体外培养机织织物或胚胎器官系统的方法,除了人类胚胎的器官系统外,还包括以下步骤:a)在每种情况下放置一个或多个在单个孔(2)的底部或底部(4)的圆形表面的中心处或附近的织物或一个或多个胚胎器官系统(6); b)在一个容器中添加完整的营养培养基(7)完全润湿井底(4)圆形表面的量是必要的; (c)在35至39°C的富含二氧化碳的潮湿气氛中孵育面料或胚器官系统(n)(6)和营养培养基(7),其特征在于,多孔板具有(1)配置变量6-孔(12-孔,24-孔,48-孔,96-孔或384-孔),并且用于不同孔板的孔(2)具有适当的井底表面(5)的格式,提供了以下体积/面积的营养培养基(7):<Table> <TableFormula> <Title /> <TableGroup colSep =“ 0” cols =“ 3” rowSep =“ 0 “> <Colspec colName =” col1“ colNumber =” 1“ colWidth =” 2 *“ /> <Colspec colName =” col2“ colNumber =” 2“ colWidth =” 2 *“ /> <Colspec colName =” col3“ colNumber =“ 3” colWidth =“ 2 *” /> <Tbody> <Row> <entry colName =“ col1”>多孔板格式</ entry> <entry>孔底表面尺寸(5),单位为cm <Sup > 2 </ Sup> </ entry> <entry colName =“ col3”>以μl为单位的营养培养基体积</ entry> </ Row> <Row> <entry colName =“ col1”> 6井</ entry> <entry colName =“ col2”> 9,6 </ entry> <entry colName =“ col3”> 816(±200 </ entry> </ Row> <Row> <entry colName =“ col1”> 12好</ entry> <entry colName =“ col2”> 3,9 </ entry> <entry colName =“ col3”> 331,5(±70 </ entry> </ Row> <Row> <entry colName =“ col1”> 24井</ entry> <entry colName =“ col2”> 1,9 </ entry> <entry colName =“ col3”> 161,5(±40 </ entry> </ Row> <Row> <entry colName = “ col1”> 48井</ entry> <entry colName =“ col2”> 1,0 </ entry> <entry colName =“ col3”> 85(±30)</ entry> </ Row> <Row> < entry colName =“ col1”> 96井</ entry> <entry colName =“ col2”> 0,32 </ entry> <entry colName =“ col3”> 27,2(±10)</ entry> </ Row > <Row> <entry colName =“ col1”> 384井</ entry> <entry colName =“ col2”> 0,1 </ entry> <entry colName =“ col3”> 8,5(±3)。< / entry> </ Row> </ Tbody> </ TableGroup> </ TableFormula> </ Table> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33">著录项</h2> <div class="getdents"> <ul> <li> <p><span>公开/公告号</span><span>DE102010043555B4</span></p> <p><span>专利类型</span><span></span></p> </li> <li> <p><span>公开/公告日</span><span>2015-07-23</span></p> <p><span>原文格式</span><span>PDF</span></p> </li> <li> <div class="apply"> <p><span style="width: 90px;display: inline-block;">申请/专利权人</span><span id="patentee" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=12_13&sertext=LEIBNIZ-INSTITUT FÜR POLYMERFORSCHUNG DRESDEN E.V.&option=232">LEIBNIZ-INSTITUT FÜR POLYMERFORSCHUNG DRESDEN E.V.;</a> </span></p> <span class="designer hoverxs" style="display: none;">展开▼</span> </div> <p><span>申请/专利号</span><span style="width: 250px;">DE20101043555</span></p> </li> <li> <div class="apply"> <p><span style="width: 90px;display: inline-block;">发明设计人</span><span id="patentee" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=12_13&sertext=CARSTEN PROF. DR. WERNER&option=202">CARSTEN PROF. DR. WERNER;</a><a href="/search.html?doctypes=12_13&sertext=DAVID D. R. SEBINGER&option=202">DAVID D. R. SEBINGER;</a> </span></p> <span class="designer hoverxs" style="display: none;">展开▼</span> </div> <p><span>申请日</span><span>2010-11-08</span></p> </li> <li> <p style="width: 100%;"><span>分类号</span><span style="width: 80%;">C12N5/071;C12N5/073;</span> </p> </li> <li> <p style="width: 100%;"><span>国家 </span><span style="width: 93%;">DE</span></p> </li> <li><p style="width: 100%;"> <span >入库时间</span> <span>2022-08-21 14:55:54</span></p> </li> </ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity"> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul > <li >专利</li> <li >外文文献</li> <li >中文文献</li> </ul> </div> <div class="similarity_details"> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120113334215.html">一种小鼠胚胎器官识别与评分方法与系统</a> <b>[P]</b> . <span> 中国专利: CN113378831A </span> <span> . 2021-09-10</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" 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