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Method and apparatus for preparing a secondary target cells and cell containing sample biological material, for extracting nucleic acids of the target cells

机译:制备次级靶细胞和含有样品生物材料的细胞的方法和装置,用于提取靶细胞的核酸

摘要

It is a method (100) for preparing a secondary target cells and cell containing sample biological material, for extracting nucleic acids of the target cells proposed. The method (100) has a step (110) of the accumulating of the target cells of the sample by separation of the target cells or the secondary cells of the sample on. Also, the method (100) a step (120) of the tenderizing of the target cells by chemical and / or physical lysis, in order to the nucleic acids of the target cells to produce cells - lysate containing. In addition, the method (100) a step (130) of the purifying of the nucleic acids from the target cells - lysate, in order to extract the nucleic acids of the target cells.
机译:这是一种方法(100),用于制备次级靶细胞和包含样品生物材料的细胞,用于提取提出的靶细胞的核酸。方法(100)具有步骤(110),该步骤(110)通过分离样品的靶细胞或二次细胞来积累样品的靶细胞。同样,方法(100)是通过化学和/或物理裂解使靶细胞嫩化的步骤(120),以使靶细胞的核酸产生含有裂解物的细胞。另外,方法(100)是从靶细胞-裂解物中纯化核酸的步骤(130),以便提取靶细胞的核酸。

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