METHOD FOR IN VITRO PRODUCTION OF THREE-DIMENSIONAL VITAL CARTILAGE OR BONE TISSUE

摘要

In vitro preparation of three-dimensional, living cartilage or bone tissue comprising culturing human or animal cartilage, bone or mesenchymal cells in vessels that have a hydrophobic surface and a tapering base, is new. In vitro preparation of three-dimensional, living cartilage or bone tissue comprises culturing human or animal cartilage, bone or mesenchymal cells in vessels that have a hydrophobic surface and a tapering base Culture is done until cell aggregates are formed, under stationary, submerged conditions. The culture contains at least 40% extracellular matrix (ECM) and has differentiated cells embedded in ECM. The aggregates include an outer region in which proliferation- and migration-competent cells are present. Independent claims are also included for the following: (a) production of cartilage and bone tissue; and (b) therapeutic composition containing the cartilage and bone tissue. - ACTIVITY : Antiarthritic; antirheumatic. No biological data provided. - MECHANISM OF ACTION : Cell replacement. No biological data provided.