NUCLEIC ACID MOLECULE ENCODING XYLOSE ISOMERASE AND XYLOSE ISOMERASE ENCODED THEREOF

摘要

The invention discloses a novel xylose isomerase nucleotide sequence obtained from a bovine rumen fluid metagenomic library and also provides the amino acid sequence encoded by the nucleotide sequence. The invention also relates to a vector and a transformant containing the nucleotide sequence. When the xylose isomerase is expressed, a host cell is endowed with the capability of converting xylose into xylulose, and the xylulose is further metabolized by the host cell. Therefore, the host cell can take the xylose as a carbon source for growth. The xylose isomerase from a new source is expressed with high activity in Saccharomyces cerevisiae and is a mesophilic enzyme with optimal temperature of 60 DEG C. The invention also relates to the applications of the xylose isomerase in theoretical research as a model protein and actual production of fuel ethanol, high fructose syrup and the like.