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A METHOD OF BIOLOGICAL TARGET DETECTION USING A NANOPORE AND A FUSION PROTEIN BINDING AGENT
A METHOD OF BIOLOGICAL TARGET DETECTION USING A NANOPORE AND A FUSION PROTEIN BINDING AGENT
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机译:一种基于纳米蛋白和融合蛋白结合剂的生物目标检测方法
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摘要
Provided are methods for detecting a target molecule or particle suspected to be present in a sample, comprising (a) contacting the sample with (i) a fusion molecule comprising a ligand capable of binding to the target molecule or particle and a binding domain, and (ii) a polymer scaffold comprising at least one binding motif to which the binding domain is capable of binding, under conditions that allow the target molecule or particle to bind to the ligand and the binding domain to bind to the binding motif; (b) loading the polymer into a device comprising a pore that separates an interior space of the device into two volumes, and configuring the device to pass the polymer through the pore from one volume to the other volume, wherein the device further comprises a sensor adjacent to the pore configured to identify objects passing through the pore; and (c) determining, with the sensor, whether the fusion molecule or particle bound to the binding motif is bound to the target molecule or particle, thereby detecting the presence of the target molecule or particle in the sample.
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