PRIMER REAGENT FOR AMPLIFYING ALK FUSION GENE, ALK FUSION GENE AMPLIFICATION REAGENT KIT INCLUDING THE SAME, AND ALK FUSION GENE AMPLIFICATION METHOD AND ALK FUSION GENE ANALYSIS METHOD USING THE SAME

摘要

The present invention aims to provide a novel primer reagent for detecting a plurality of variants of the ALK fusion gene easily through gene amplification in one reaction system. The primer reagent for an ALK fusion gene according to the present invention includes: a forward primer consisting of the sequence of the following oligonucleotide (F_V1/6); a forward primer consisting of the sequence of the following oligonucleotide (F_V2); a forward primer consisting of the sequence of the following oligonucleotide (F_V3a); a forward primer consisting of the sequence of the following oligonucleotide (F_V3b); a reverse primer consisting of the sequence of the following oligonucleotide (R_V/multi); and a reverse primer consisting of the sequence of the following oligonucleotide (R_V6), wherein (F_V1/6) is an oligonucleotide consisting of the base sequence of SEQ ID NO: 1; (F_V2) is an oligonucleotide consisting of the base sequence of SEQ ID NO: 2; (F_V3a) is an oligonucleotide consisting of the base sequence of SEQ ID NO: 3; (F_V3b) is an oligonucleotide consisting of the base sequence of SEQ ID NO: 4; (R_V/multi) is an oligonucleotide consisting of the base sequence of SEQ ID NO: 5; and (R_V6) is an oligonucleotide consisting of the base sequence of SEQ ID NO: 6.