Selection system containing non-antibiotic resistance selection marker

摘要

The present invention relates to a novel selection system, which is based on the use of an araD gene, a mutated form of an araD gene, a complementary sequence thereof, or a catalytically active fragment thereof as a selection marker and to the use of a bacterial strain deficient of the araD gene. The present invention further relates to novel vectors containing an araD gene, a mutated form of an araD gene, a complementary sequence thereof, or a catalytically active fragment thereof and to novel bacterial strains deficient of the araD gene. The present invention additionally relates to a method of selecting the cells transformed with a plasmid, which contains the gene of interest.