PRODUCTION METHOD OF CARDIOMYOCYTE, VENTRICULAR TYPE CARDIOMYOCYTE AND PRODUCTION METHOD THEREOF, AS WELL AS SCREENING METHOD

摘要

PROBLEM TO BE SOLVED: To provide a production method of a cardiomyocyte capable of producing a cardiomyocyte of high quality, to provide a ventricular type cardiomyocyte of high quality in which the contamination of a non-cardiomyocyte and the heterogeneity of a cardiomyocyte are reduced, and a production method thereof, as well as to provide a screening method of a preventive or a therapeutic agent for at least one of hypertrophic cardiomyopathy, cardiac hypertrophy, and Noonan syndrome using at least one of the cardiomyocyte and the ventricular type cardiomyocyte.SOLUTION: A production method of a cardiomyocyte and the like comprises: an embryoid formation step of culturing pluripotent stem cells to form an embryoid; a mesoderm induction step of culturing the embryoid to induce a mesoderm; a cardiomyocyte induction step of culturing the embryoid after the mesoderm induction to induce a cardiomyocyte; and an embryoid dissociation step of dissociating the embryoid after the cardiomyocyte induction. The embryoid dissociation step is performed during a period from the start of the mesoderm induction step to the 40th day.SELECTED DRAWING: None