IMPROVED METHOD FOR FORENSIC DNA QUANTIFICATION

摘要

PROBLEM TO BE SOLVED: To provide methods for quantifying human genome double stranded DNA target.SOLUTION: The invention relates to a quantitative test method for a test liquid, which contains or is presumed to contain a target nucleic acid in a micro fluid channel, comprising: a step of not amplifying the target in a sample in the step of forming the test liquid by combining a test fluid and a molecule beacon having a signaling portion capable of binding to human Alu sequence; a step of denaturing double stranded DNA by heating and cooling the sample fluid to form the test liquid by combining with a molecule beacon probe; a step of binding the molecule beacon probe to the target; a step of detecting a signaling portion in a detection region in the micro fluid channel; and a step of quantifying the target in the sample fluid within one minutes from combining the sample fluid and the molecule beacon probe.SELECTED DRAWING: None