Occurrence of pH / temperature / ion gradient in the lateral flow immunoassay for adjusting the biomolecular interactions and its applications

摘要

a lateral flow assay device comprising a multi-site array of test sites for measuring a biomolecule analytes (3) (8), lateral flow assay device, absorbent materials to provide a capillary flow has a solid support (5), including, one device is divided into parallel lane (7) in the flow direction. Device includes a sample portion for receiving a sample, a diagnostic moiety, including multi-site array of test sites each comprising a probe (8) for said biomolecule analytes (3), of each of the multi-site array in the test site, the condition on the interaction between the biomolecule sample and the probe may be varied independently, diagnostic moiety, the absorption material portion of the absorbent material for providing a capillary flow, hydraulic It is allowed, and an electrode (10) for controlling the condition. The sample moieties, diagnostic moieties, and absorbent material portion is in communication with capillary flow, causing capillary flow directed from the sample portion in the absorbent material portion as a result, whereby, the sample is, in the above diagnostic moieties in each test site, flows across the probe, providing a contact between the sample and such probes. Using such a lateral flow assay device is a method for measuring the analyte concentration in the sample.FIELD 2