Fluorescent dot counting method in the digital pathology image

摘要

fluorescence in situ hybridization (FISH), specific DNA sequences in the cell chromosome by use of a selective staining It allows for detection. Due to the high sensitivity, FISH can simultaneously detect a plurality of targets, using multiple colors. Target signal is represented as a colored dot, the counting of these signals is referred to as the dot count. The use of two-stage segmentation framework, the dot every localization that may contain the overlapping dots is guaranteed.BACKGROUND 1