High throughput HPV immunoassays that are based on the cells for the treatment and screening of HPV-associated cancers

摘要

Method of quantitating the expression of HPV proteins in clinical samples are disclosed. Obtaining clinical samples include quantification method. Clinical samples such comprised of a population of cells that are susceptible to infection by HPV. The quantitative method includes the steps of attaching the sample container is included. Under conditions that promote specific binding of the primary antibody to HPV proteins expressed by the population of cells, I contacting the clinical sample with an antibody that specifically binds to an HPV protein expressed by HPV-infected cells. Furthermore, to quantify the specific binding of the primary antibody, a step of quantifying the expression of HPV proteins in clinical samples are included whereby the method. Assay provides an objective test to identify patients with aura of high grade sample from cytology biopsy before. [Selection Figure FIG 1A~1C