首页> 外国专利> PRODUCTION OF DIRHAMNOSE-LIPID IN RECOMBINANT NONPATHOGENIC BACTERIUM PSEUDOMONAS CHLORORAPHIS

PRODUCTION OF DIRHAMNOSE-LIPID IN RECOMBINANT NONPATHOGENIC BACTERIUM PSEUDOMONAS CHLORORAPHIS

机译：重组非致病性铜绿假单胞菌中脂多糖的生产

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摘要

Pseudomonas chlororaphis NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C_12:1-C₁₀) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C₁₂-C₁₀) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (R₁L) is described. Expression of two of these genes, i.e., rhlA and rhlB, together in P. chlororaphis NRRL B-30761 increases R₁L production by at least 10-fold. Also the generation of a recombinant P. chlororaphis NRRL B-30761 capable of synthesizing dirhamnose-lipid (R₂L) is described. Characterization of R₁L and R₂L produced by the recombinant P. chlororaphis NRRL B-30761 is also described.

机译： Pseudomonas chlororaphis NRRL B-30761产生单鼠李糖脂，其中3-羟基十二碳烯基-3-羟基癸酸酯（C _{12：1 -C _{10 ）或3-静态生长条件下，羟基十二烷酰基-3-羟基癸酸酯（C _{12 -C _{10 ）为脂质部分。描述了与鼠李糖脂（R _{1 L）生物合成相关的三个基因和蛋白质的克隆和测序。这些基因中的两个基因，即rhlA和rhlB，一起在 P中表达。 chlororaphis NRRL B-30761使R _{1 L的产量增加至少10倍。还产生重组 P。描述了能够合成二甘露糖-脂（R _{2 L）的草绿 NRRL B-30761。重组 P产生的R _{1 L和R _{2 L的表征。还描述了草绿 NRRL B-30761。

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公开/公告号US2016102330A1

专利类型

公开/公告日2016-04-14

原文格式PDF

申请/专利权人 THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF AGRICULTURE;
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申请/专利号US201414892793

发明设计人 RICHARD D. ASHBY;NEREUS W. GUNTHER;DANIEL SOLAIMAN;JONATHAN A. ZERKOWSKI;
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申请日2014-06-03

分类号C12P19/44;C12P7/64;C12N9/10;

国家 US

入库时间 2022-08-21 14:38:42

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