Fluorogenic/Fluorescent Probes Derivative from Sulfoxanthene, and Use Thereof

摘要

The invention relates to fluorescent/fluorogenic probes of formula (I″) or (II″):; ;wherein Z is chosen between:—NH₂ and —OH,or in the fluorescence quencher group consisting of:—NO₂;—N═N—R¹; R¹ being any organic group that does not obscure the corresponding azo bond;—NHCO-Pept.; Pept. being a peptide residue or any organic group that does not obscure the corresponding amide bond;—O-Glyc.; Glyc. being a oligoglycoside residue that does not obscure the corresponding glycosidic bond;—O—C(O)—R²; —O—P(O)(OR²)(OR²′) and —O—S(O)₂—R²; R² and R²′ being independently a hydrogen atom or an organic group that does not obscure the corresponding ester bond; and; R_a, R_b, R_c, R_d and R_e being independently a hydrogen atom or any organic group that does not obscure the corresponding arylether bond so as not to prevent its possible cleavage by a myeloperoxidase activity. ;It also relates to the use of these fluorescent/fluorogenic probes, for the detection of an enzyme activity, notably in order to identify/discriminate microorganisms in function of their ability to express particular enzyme activities.