METHODS FOR GENERATING 3D CELL CULTURE MODEL IN HIGH RESPIRATION ENVIRONMENTS FOR HIGH-CONTENT ANALYSES AND SCREENING

摘要

A method of culturing cells to form cell aggregations and subjecting the cell aggregations to imaging analysis, comprises: seeding cells in a culture medium on a substantially flat substrate that is transparent, oxygen gas-permeable and liquid impermeable and is coated with atelo-collagen of large-mammalian origin; culturing the cells by continuously supplying air or oxygen-containing gas to the cells through said substrate to form the cell aggregations that are three-dimensional (3D), shaped as undulated as islands or mountains and discretely arranged as islands separated by substantially cell-free gaps; subjecting the cell aggregations on said substrate to the imaging analysis by using lights passing through said substrate.